Introduction to chromatography lab activity
Students will separate a dye mixture using polar and non-polar solvents instead of petroleum ether/ acetone solvent. They will also use a non-polar solvent to separate out the various pigments found in leaves and learn the function of each pigment during photosynthesis. The kit comes with a chlorophyll extract, so you don’t have to waste time preparing one. It also includes materials for ten setups, teacher’s guide, and student copymaster. The demonstration size has enough materials for five demonstrations.
Materials Included
•1 Vial of plant pigment extract, 2 mL (chlorophyll solution)
•1 Vial of chromatography dye mixture, 2 mL
•1 Bottle of chromatography developer II (Isopropyl alcohol)
•1 Bottle of chromatography developer III (Isopropyl/ water)
•1 Bottle of chromatography developer IV (Methanol/ water)
•1 Capillary sample loading tubes, pkg. 50
•50 Chromatography paper strips
•30 Chromatography reaction chambers
•1 Teacher’s Guide
•1 Student Study and Analysis Sheet (Copymaster)
Materials Needed But Not Provided
•[Chromatography developer I (Water)]
Time Requirement
•30 Minutes