Chromatography of spinach
The procedure presented in this activity quickly yields large amounts of plant pigments, making it easier to complete than traditional solvent extractions. Students will learn the basics of paper chromatography, including the use of petroleum ether/acetone solvent, and calculating the rate of flow (Rƒ) for each pigment. Once students have completed the experiment they will be rewarded with a vivid chromatogram displaying chlorophyll a and b, xanthophyll, and carotene pigments, as well as an understanding of the different affinities for the mobile and stationary phases. The kit includes enough materials (you provide the spinach) for 15 setups, a teacher’s guide, and student copymaster. Note: It is recommended that this procedure be performed under a fume hood.
Materials Included
•15 Chromatography vials with caps
•20 Chromatography strips
•15 Disposable pipets
•15 Metric rulers
•4 Bottles, chromatography solvent
Materials Needed But Not Provided
•15 Fresh spinach leaves
•15 Coins (ribbed coins preferably)
•Pencils
•Scissors
•Forceps
•Chemical fume hood
•Gloves
•Apron