Family pedigree cancer gene detection
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In this experiment, students determine a pedigree for a family thought to be carriers of a mutation in their p53 genes. This is followed by a diagnostic agarose gel analysis to diagnose the state of the p53 gene in individual family members. Kit includes: instructions, DNA samples, agarose, running buffer, InstaStain DNA stain, practice gel loading solution, 1mL calibrated drop pipette, 100mL graduated cylinder and microtipped transfer pipettes. All you need: electrophoresis tank, power supply, micropipettes (550 μL adjustable or 40 μL fixed volume) and a white light box is recommended